1 00:00:04,470 --> 00:00:03,750 hello thank you for checking out my 2 00:00:06,230 --> 00:00:04,480 lightning talk 3 00:00:07,990 --> 00:00:06,240 i'm rachel tucker an undergraduate 4 00:00:09,509 --> 00:00:08,000 student at the university of florida 5 00:00:11,110 --> 00:00:09,519 and i've been studying martian regolith 6 00:00:12,470 --> 00:00:11,120 as a substrate for foreign planetary 7 00:00:14,789 --> 00:00:12,480 horticulture 8 00:00:16,470 --> 00:00:14,799 why do regular studies matter human 9 00:00:17,590 --> 00:00:16,480 space exploration is increasing in 10 00:00:19,349 --> 00:00:17,600 duration and distance 11 00:00:20,950 --> 00:00:19,359 which poses a greater need for in-situ 12 00:00:22,950 --> 00:00:20,960 resource utilization in order to 13 00:00:25,109 --> 00:00:22,960 maximize available resources 14 00:00:26,470 --> 00:00:25,119 a regolith simulant offers a comparable 15 00:00:28,470 --> 00:00:26,480 substrate in order to study the 16 00:00:30,870 --> 00:00:28,480 potential of martian regolith as a local 17 00:00:32,229 --> 00:00:30,880 resource for crop production on mars 18 00:00:33,910 --> 00:00:32,239 why do we want to look at mars global 19 00:00:35,590 --> 00:00:33,920 simulant 1. 20 00:00:36,950 --> 00:00:35,600 other martian simulants are available 21 00:00:39,190 --> 00:00:36,960 such as jsc mars 1 22 00:00:40,630 --> 00:00:39,200 and mojave mars simulink both are 23 00:00:42,869 --> 00:00:40,640 sourced from natural environments and 24 00:00:45,190 --> 00:00:42,879 have been widely studied in literature 25 00:00:46,869 --> 00:00:45,200 mars global simulant 1 is a recipe 26 00:00:49,510 --> 00:00:46,879 created by excellence labs 27 00:00:51,270 --> 00:00:49,520 to mimic the mineral chemical volatile 28 00:00:52,790 --> 00:00:51,280 and spectral properties of the rockness 29 00:00:56,150 --> 00:00:52,800 regolith at gale crater which is the 30 00:00:58,869 --> 00:00:56,160 best characterized regolith to date 31 00:00:59,189 --> 00:00:58,879 in our experiment we wanted to determine 32 00:01:02,709 --> 00:00:59,199 what 33 00:01:03,349 --> 00:01:02,719 low concentrations of the mars global 34 00:01:06,310 --> 00:01:03,359 stimulant 35 00:01:06,870 --> 00:01:06,320 how that could affect crops and so we 36 00:01:08,469 --> 00:01:06,880 tested 37 00:01:10,310 --> 00:01:08,479 three different concentrations of 38 00:01:13,830 --> 00:01:10,320 regolith relative 39 00:01:17,429 --> 00:01:13,840 to an inert arcellite substrate base 40 00:01:19,270 --> 00:01:17,439 we tested 10 25 and and 50 percent 41 00:01:20,550 --> 00:01:19,280 mars global stimulant one relative to 42 00:01:23,030 --> 00:01:20,560 the arcellite 43 00:01:24,789 --> 00:01:23,040 during growth we we collected daily 44 00:01:26,870 --> 00:01:24,799 collected data daily for 45 00:01:28,710 --> 00:01:26,880 radical emergence cotyledon emergence 46 00:01:30,870 --> 00:01:28,720 first true leaves budding 47 00:01:33,030 --> 00:01:30,880 and at 10 days after planting we 48 00:01:37,270 --> 00:01:33,040 harvested and collected fresh weight 49 00:01:39,590 --> 00:01:37,280 dry weight height and widest diameter 50 00:01:40,870 --> 00:01:39,600 we planted 25 seeds for each of the 51 00:01:43,590 --> 00:01:40,880 following cultivars 52 00:01:44,550 --> 00:01:43,600 daikon radish amara mustard extra dwarf 53 00:01:46,630 --> 00:01:44,560 bok choy 54 00:01:49,270 --> 00:01:46,640 buckwheat red russian kale and 55 00:01:52,389 --> 00:01:49,280 outrageous red romaine 56 00:01:55,590 --> 00:01:52,399 pictured here is a top view of 3 57 00:01:57,510 --> 00:01:55,600 5 7 and 10 days after planting of the 58 00:02:01,429 --> 00:01:57,520 control group in the top row 59 00:02:03,910 --> 00:02:01,439 10 25 and the 50 concentration of mars 60 00:02:06,069 --> 00:02:03,920 global stimulant 61 00:02:07,990 --> 00:02:06,079 as you can see as the concentration of 62 00:02:12,229 --> 00:02:08,000 mars global stimulant increases the 63 00:02:15,990 --> 00:02:13,750 picture here is a graph for each of the 64 00:02:17,190 --> 00:02:16,000 cultivars and the x-axis is the number 65 00:02:19,830 --> 00:02:17,200 of days after planting 66 00:02:21,110 --> 00:02:19,840 and in the y-axis is the percent of 67 00:02:22,470 --> 00:02:21,120 seeds planted 68 00:02:24,229 --> 00:02:22,480 as you can see here for radical 69 00:02:25,910 --> 00:02:24,239 emergence there was a large difference 70 00:02:27,030 --> 00:02:25,920 in each of the six cultivars between the 71 00:02:28,949 --> 00:02:27,040 tony 25 72 00:02:31,990 --> 00:02:28,959 concentration of regolith and the 50 73 00:02:36,309 --> 00:02:35,509 for cotyledon emergence in five of the 74 00:02:37,509 --> 00:02:36,319 six 75 00:02:39,430 --> 00:02:37,519 cultivars with an exception of 76 00:02:40,390 --> 00:02:39,440 outrageous red romaine there was a large 77 00:02:43,030 --> 00:02:40,400 difference between the 78 00:02:45,030 --> 00:02:43,040 10 and the 25 percent concentration of 79 00:02:46,550 --> 00:02:45,040 regolith 80 00:02:48,630 --> 00:02:46,560 and again for the first true leaves 81 00:02:49,990 --> 00:02:48,640 budding for five of the six cultivars 82 00:02:51,509 --> 00:02:50,000 the exception of the outrageous red 83 00:02:52,229 --> 00:02:51,519 romaine there's a large difference 84 00:02:55,509 --> 00:02:52,239 between 85 00:02:58,149 --> 00:02:55,519 the 25 and the 10 concentrations of the 86 00:03:01,110 --> 00:02:59,430 at harvest there was not enough 87 00:03:01,830 --> 00:03:01,120 vegetative tissue in order to collect 88 00:03:05,110 --> 00:03:01,840 data for 89 00:03:07,830 --> 00:03:05,120 the 50 concentration of regolith however 90 00:03:09,589 --> 00:03:07,840 for the 20 fresh weight for the 25 91 00:03:11,910 --> 00:03:09,599 concentration of regolith relative 92 00:03:12,710 --> 00:03:11,920 to the control group each of the six 93 00:03:14,149 --> 00:03:12,720 cultivars 94 00:03:17,030 --> 00:03:14,159 was statistically significant in 95 00:03:21,589 --> 00:03:19,910 for dry weight five of the six cultivars 96 00:03:23,030 --> 00:03:21,599 were statistically significant between 97 00:03:24,149 --> 00:03:23,040 the twenty-five percent concentration of 98 00:03:27,509 --> 00:03:24,159 burglars and the test 99 00:03:31,509 --> 00:03:29,589 for the height for the harvest data it 100 00:03:33,589 --> 00:03:31,519 was very statistically significant in 101 00:03:35,190 --> 00:03:33,599 terms of the 25 percent 102 00:03:36,630 --> 00:03:35,200 concentration of regolith in which all 103 00:03:37,509 --> 00:03:36,640 six cultivars were statistically 104 00:03:40,470 --> 00:03:37,519 significant 105 00:03:42,229 --> 00:03:40,480 and in 10 concentration of regolith five 106 00:03:43,030 --> 00:03:42,239 of the six cultivars were statistically 107 00:03:44,710 --> 00:03:43,040 significant 108 00:03:47,270 --> 00:03:44,720 and as you can see pictured here the 109 00:03:49,110 --> 00:03:47,280 extra prok choi at zero percent 110 00:03:53,830 --> 00:03:49,120 um or the control group and the 10 111 00:03:57,110 --> 00:03:56,789 diameter was also greatly affected in 112 00:04:00,229 --> 00:03:57,120 for 113 00:04:01,429 --> 00:04:00,239 all six cultivars the 25 percent 114 00:04:02,869 --> 00:04:01,439 concentration of regolith was 115 00:04:04,869 --> 00:04:02,879 statistically significant from the 116 00:04:07,190 --> 00:04:04,879 control group 117 00:04:08,309 --> 00:04:07,200 what does this mean unamended mars 118 00:04:10,869 --> 00:04:08,319 global stimulant 1 119 00:04:12,550 --> 00:04:10,879 severely inhibits vegetative crop growth 120 00:04:15,030 --> 00:04:12,560 in multiple cultivars 121 00:04:17,990 --> 00:04:15,040 and not just at 100 concentration this 122 00:04:21,349 --> 00:04:18,000 occurs at low concentrations as well 123 00:04:23,270 --> 00:04:21,359 and what this means is that this this 124 00:04:25,830 --> 00:04:23,280 regolith will need to be altered in some 125 00:04:29,189 --> 00:04:25,840 way in order to become a viable 126 00:04:31,990 --> 00:04:29,199 resource for crop growth on mars 127 00:04:32,629 --> 00:04:32,000 of course this was not done as one 128 00:04:34,710 --> 00:04:32,639 person 129 00:04:36,390 --> 00:04:34,720 science takes a lot of people and a lot 130 00:04:36,950 --> 00:04:36,400 of wonderful people contributed to this 131 00:04:39,350 --> 00:04:36,960 product